extraction de phosphate d'inositol à partir de graines de coton en Haïti

                                               
                                               
                                               
                                               
  • extraction de phosphate d'inositol à partir de graines de coton en Haïti

Can inositol be produced without adenosine triphosphate supplementation?

In order to produce inositol from glucose without adenosine triphosphate (ATP) supplementation, a tri-enzymatic cascade pathway has been delivered, where polyphosphate glucokinase from Arthrobacter sp. has aided the phosphorylation to glucose-6-phosphate. IPS, a rate-limiting enzyme, was chosen from Trypanosoma brucei.

How to extract myo-inositol from agricultural sources?

Although a number of different extraction routes such as conventional, microwave, ultra – sonication, and pressurized-liquid are widely investigated for the recovery of myo -inositol from agricultural sources but, there is still space for improvement in the extraction yield for commercial scale production.

How is myo inositol extracted?

Myo- inositol is obtained industrially by either chemical hydrolysis or microbial fermentation (Li et al., 2022). Extraction of myo-inositol is usually done conventionally by organic solvents like ethanol and methanol, with different proportions of water under a varied range of time-temperature combinations to be optimized.

How is myo-inositol extracted from dragon fruit?

Myo-inositol from dragon fruit is extracted with 60% ethanol (Rebecca et al., 2012). Myo-inositol content is found in cocoa beans by extracting it with 80% alcohol (Megías-Pérez et al., 2019).

Is microwave-assisted extraction a non-conventional method for inositol extraction?

Recently, there has been considerable usage of microwave-assisted extraction (MAE) as a non-conventional method for extraction of inositol, where non-ionizing microwaves in the range of 300 MHz to 300 GHz, are used to elevate the temperature of solvents in contact with components in a microwave extraction cell (Fig. 4).

How is inositol produced by Loewus pathway?

From glucose-1-phosphate and glucose-6-phosphate, inositol is produced by Loewus pathway with the help of MIPS enzyme. For plasmid construction, E. coli TOP10 was utilized. 50 g/L of cello-dextrin produces 36.3 g/L of inositol within 36 h (Meng et al., 2018).